In Vitro Fertilization (IVF): the steps

IVF takes place very methodically, in several stages. These stages are not necessarily always the same according to the teams, or the women to be treated. This treatment varies from 30 to 15 days depending on the protocol used.

Here are the different stages of IVF:

Hormonal treatment

The woman will receive hormone therapy (GnRH agonist) to stop the control of the brain on the ovaries and to stimulate the latter directly.

The stimulation

Different hormonal treatment (FSH) stimulates the follicles. Once the follicles have matured, ovulation is triggered by another hormone injection (HCG).

Several ultrasounds are performed to evaluate the response of the ovaries and measure the evolution of follicle size.


Under local or general anesthesia, the ripe oocytes are removed by means of a small tube and a needle which is introduced into the vagina. This procedure is used to puncture the follicular fluid which is then placed in a tube containing a suitable nutrient liquid at a temperature of 37 ° C.

The oocytes present in the follicular fluid are recovered and the sperm is collected a few hours earlier (or thawed the same day). Spermatozoa are separated from seminal fluid and stored at 37 ° C as well.


For classical IVF : Spermatozoa and oocytes are put together in a nutritious liquid for several hours at body temperature; fusion, fertilization then occurs spontaneously.

For IVF ICSI (intracytoplasmic injection), the selected sperm is directly introduced into the oocyte.

The development of the embryo and its transfer

The egg begins to multiply and when there are four cells, it is examined under a microscope, and can be transferred to the uterus of the woman using a small tube. One or more embryos can also be preserved through freezing.

The egg starts to multiply and 2-3 days later, when there are four cells, it is examined under a microscope: if it meets the required criteria, it can be transferred to the woman's uterus. using a small tube slipped vaginally.

The embryo or embryos then deposited develop until they implant in the uterine lining for the pregnancy to continue.

One or more embryos can also be stored for a next attempt through freezing.

You want to react, to give your testimony or to ask a question? See you in our FORUMS Getting pregnant, Infertiveness or A doctor answers you!

Read also :

> Testimonials on artificial insemination
> Ovarian stimulation, instructions for use
> Egg donation: how is it going?
> Artificial insemination: they testify
> Why can not I get pregnant?
> GPA: the debate on surrogates

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